IGEM:IMPERIAL/2007/Notebook/2007-9-24

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<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Purification of LuxR

  • On Friday we harvested cells produciong LuxR and collected the inclusion bodies. Today we carried out refolding and ran an affinity column to seperate out the His-LuxR
  • At the end of the experiment we ended up with several fractions, when the elution profile was looked at there was only two peaks, one very slight one and one very step one for the last fraction
  • We will run a gel tomorrow to determine where the LuxR