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<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/20 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Purification of LuxR-His

  • Today we innoculated 2 litres of LB medium with E.coli containing the luxR-his plasmid (pMU117) to be grown to an O.D.600 0.5. After this the cells were harvested and frozen overnight.
  • In addition an SDS-PAGE gel was run from a small sample of the soluble proeins from these cells, that had been purified using an affinity column (Nickel column for His tag).
  • The gel was left to destain overnight