IGEM:IMPERIAL/2007/Notebook/2007-8-9

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Cloning of Biobricks

1. Placed 5 ml of LB in a 15 ml tube
2. Added appropriate antibiotics into the tube
3. Picked a colony from the fresh overnight plate
4. Innoculated the colony in the LB media
5. Grew overnight at 37°C in shaking incubator
   • 2x 14 Biobricks cloned