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<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/2 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Preparation of Stock Solutions

  • 1x 1 litre of 1M Tris-Cl
  • 1x 500 ml of 50x Tris-acetate

Preparation of Competent Cells

  1. Picked colonies from agar plates
  2. Grew colonies in LB media
    • DH5Alpha
    • XL1Blue
  3. Incubated cultures at 37°C overnight