IGEM:IMPERIAL/2007/Notebook/2007-10-17

Temperature step test for pTet-GFPmut3b

• The aim of this test was to determine the response of the pTet-GFPmut3b construct to a step change in temperature.
• For complete materials and methods see the Protocol
• The construct was tested at two temperature steps:
  1. From 4°C (for 3 hours) to 20°C (3 hours)
  2. From 20°C (for 3 hours) to 4°C (3 hours)
• The sampling time was every hour
• The Results show that the step changes in temperature affect the fluorescence produced by the pTet-GFPmut3b construct