IGEM:IMPERIAL/2007/Calendar/2007-9-3

We are in Week 9 of the iGEM project

Project Calendar

<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Debrief

Testing of DNA constructs

• Raw results up on the wiki
• Refinfed data and graphs currently being put up
• Data to be given to modelling team for analysis
• Problems with staggering of experiments - results not very useful
  • Talk to supervisors
  • Try to get longer access to equipment (fluorometer, incubator etc)
  • Find out methods to reduce error and variability in results

Cloning

• pTet and pLux constructs have been cloned
  • > 300µg of each DNA made - enough for all experiments
• LuxR arrived - might be transformed by Wed
  • Protocol team to talk to clonning team about details
• pT7-GFP construct being cloned in BL21

Vesicles

• New procedure being adapted which works well- not leaving overnight and using 10ml suspension
  • Saves ingredients
  • No need to leave overnight
• Cell extract will be tested in vesicles tomorrow
• FACS machine for counting vesicles - not sure if can get access
• No concrete decision yet made on where vesicles fit into the project
  • Meeting between Dirk (captain vesicles), Ben (captain ID) and Alex (captain CBD) to discuss feasibility of vesicles

Wiki

• In the process of being redesigned - Alex and Anthony
  • Make wiki more organise and facilitate navigation for visitors
  • Talked to Vincent
  • Talking to individual groups about sections relevant to them and proposed layout

AOB

• Characterisation of chassis - need to focus on it a bit more
  • Modelling and testing within in-vitro and vesicles
  • Use literature rather than experiments to characterise chassis being used
  • Use the information in in-veso teams(old oraganisation) page