IGEM:IMPERIAL/2007/Calendar/2007-8-30

We are in Week 8 of the iGEM project

Project Calendar

<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Debrief

Vesicles

Should the testing and characterisation of vesicles be pursued?

Reasons to abandon

• Process of formation not very predictable - not even with same protocols
  • variation would affect characterisation
• May not be able to carry out all tests for the applications
  • not enough time or resources (finances and equipment)
• No method of counting vessicles - but can get less accurate results by population analysis
• Found non-toxic pore protein - expensive
• ID lagging in terms of testing of constructs - can use the man power in the lab
• Would need a lot more man power to carry on research and do experiments - may still not finish on time!

Reasons to pursue

• GFP may degrade after a few hours in-vitro - vessicles would increase life-span to ~ 3-4 days
  • life-span of at least a few days required for the CBD construct to fulfil current specification
  • but can modify the specification - threshold device rather than thermal history?
• Haven't yet tried the cell extract in vessicles - may get better results!

Experiments

• Phase 2 started - testing CBD constructs for temperature
• Experiments going ok - but may run over in terms of the budget
• ID construct - purification of LuxR may take time

Wiki

• Tidy up 'public wiki' - seperate from 'working wiki'
• Protocols hard to understand for outsiders - make more readable
• Calender??
• Anthony and Alex to re-design the wiki