We are in Week 8 of the iGEM project
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Should the testing and characterisation of vesicles be pursued?
Reasons to abandon
- Process of formation not very predictable - not even with same protocols
- variation would affect characterisation
- May not be able to carry out all tests for the applications
- not enough time or resources (finances and equipment)
- No method of counting vessicles - but can get less accurate results by population analysis
- Found non-toxic pore protein - expensive
- ID lagging in terms of testing of constructs - can use the man power in the lab
- Would need a lot more man power to carry on research and do experiments - may still not finish on time!
Reasons to pursue
- GFP may degrade after a few hours in-vitro - vessicles would increase life-span to ~ 3-4 days
- life-span of at least a few days required for the CBD construct to fulfil current specification
- but can modify the specification - threshold device rather than thermal history?
- Haven't yet tried the cell extract in vessicles - may get better results!
- Phase 2 started - testing CBD constructs for temperature
- Experiments going ok - but may run over in terms of the budget
- ID construct - purification of LuxR may take time
- Tidy up 'public wiki' - seperate from 'working wiki'
- Protocols hard to understand for outsiders - make more readable
- Anthony and Alex to re-design the wiki