IGEM:IMPERIAL/2007/Calendar/2007-8-28
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We are in Week 8 of the iGEM project
Project Calendar
<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Debrief
Experiments
Phase 1
Inital testing of the biobricks
- pTet works
- doesn't work below 10°C or above 45°C
- fluorescence peak at 25°C - needed a monotonic relation between temperature and GFP expression
- pT7 - doesn't work - being re-cloned
- pLux - works at quite a high [AHL]
- not very good fluorescence for a realistic [AHL]
- no GFP for 1nM of AHL
- possible to change the threshold of the construct?
Cell extract testing
- Home-made cell extract with home-made reaction buffer - doesn't work
- Flooded the extract with DNA - still dosn't work
- Need more comercial cell extract - budget limit almost reached
- Need to plan experiments more efficiently
Modelling
- Finished models for construct 1 and 2
- Currently documenting on the wiki
- Preparing for presentation
Vessicles
- Prepared presentation for Wednesday
- Done calculations for changes in protocols - to make the development of vessicles better
- Test again tomorrow
- Might have found a non-toxic method to create pores on vessicles - need further investigation
- Will affect stability and life-span
Cloning of biobricks
- All working biobricks cloned
- Making substitutes for the ones not woorking
- New parts to be added to the registry
- Waiting for dsRed to arrive
- No further ligations
- Purified LuxR - has been ordered (USA)
- Will pursue both construct 1 and 2 for ID