IGEM:IMPERIAL/2007/Calendar/2007-8-24
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We are in Week 7 of the iGEM project
Project Calendar
<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Debrief
Modelling
- Simulations almost completed - will be put on the wiki during the weekend
- Compared the modelled graph of pLux construct with the one got by experiment - have the same general shape but no peek in the model
- ID construct - problem with the constitutive promoter - need more data
Vesicles
- Have small vesicles with GFP - have more in quantity than before but not entirely sure if they are actually vessicles
- Need to decide whether to concentrate on gene expression or proof of concept - need more people in the vesicles team
Lab team
- Finished makig the reaction mixture
Protocols
- Tested homemade cell extract with the reaction buffer - doean't work
- LuxR construct - works quite well in-vitro
- Will test comercial cell extract with the homemade reaction buffer
Meeting with Prof. Kitney
- Make presentations - make them see the big picture
- Need to confirm length of meeting
- Presentation teams:
- Veso - Dirk, Alex
- CBD - Alex, PPX, Ant, Lucas
- ID - Ben, Jerry, Maira, James
- Logo - Dirk, Ant