IGEM:IMPERIAL/2007/Calendar/2007-8-20

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We are in Week 7 of the iGEM project

Project Calendar

<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Debrief

Modelling

  • Got most equations for infector detector - up on the wiki
  • Most simulations done
  • Some problems, like with the constitutive promoter - will discuss with Captain ID and protocols team to resolve the issues
  • Need to finish forming all the equations and highlight all the issues with the modelling of both ID and CBD by the end of the week

Vessicles

  • Didn't form any vessicles today
  • Got vessicles for sure, but GFP seems to be only around the membrane - possible reason: GFP was not put in the right quantity at the right step
  • Will need to form vessicles again to verify

Protocols

  • Reaction buffer needed for the home-made cell extract to work - ordered all the reagants
  • Home-made cell extract tests put on hold - will carry on testing the DNA constructs in the commmercial one
  • Tests will be done for pTet, PT7, GFP in vitro

Lab team

  • IPTG solution made for the cell extract experiments
  • Cloned a few more bricks, and re-cloned some that didn;t work earlier

Weekly digest

  • Done and send to all the supervisors