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The aim of this experiment was to make a calibration curve to be able to convert Abs490, which is measured by the plate reader, into OD600.
- A dilution series of different OD600s was created using the in-lab spectrophotometer and overnight culture of cells.
- These dilutions were also assessed via the spectrophotometer to make sure they were near their targetted OD.
- 3 x 200μL of each dilution was pipetted into a 96 well plate and then Abs490 was read by the Victor3 plate-reader
- The cells and the dilutions were kept on ice to prevent any increase in OD
- Data File: