Discussion with Tom
fews things need to be cleared asap:
- Which medium to be used in testing + final experiment, LB or M9 ? We have to take different things into account:
- Fluorescent backround
- Growth rate for the culture
- Medium should allow aiiA activity (Zinc hypothesis)
- Medium should allow AHL production
- Some quick experiments should be designed and done.
- The Victor 3 equipment only allows OD 490nm absorbance, we should check what is the difference with OD600 (growth curves).
- What is the impact of GFP production on growth rate in T9002 ?