IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/30
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LTP ihpRNA parts that worked: 9c2, 11c1, 11c2
Bet 2 ihpRNA parts that worked: 25c1,c2, 28c1,(LTPS+PDK is actually Bet 2 hpRNA)
Ger ihpRNA parts that worked: 36c1,c2, 37c1,2, 38c1,c2
Ligations were plated and left @ 37°C O/N.
PCR of VP16 and Gal4DBD
PCR again for VP16
Using pfx polymerase
Running PCR on two reactions. There was not enough PFX for both to get .5μL, so only one of them is likely to actually amplify.
Second ACC + RxrHm digest gel
yes to ACC (left) no RxrHm