IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week8/Widgetry

Goals for this week

1. Run co-culture test with DH5α, DH5α/pUC19, and Shewie
2. Move chambers to the "dark" to prevent a reaction from happening with light and oxygen
3. Test light-repressible system on plates

Monday: August 11, 2008

• cleaned chambers
• moved setup to a cabinet to have system in the "dark"

DH5α, DH5α/pUC19, and Shewie co-culture experiment setup

• Chamber 1: DH5α + wt Shewie + Lactose
• Chamber 2: DH5α + wt Shewie + Lactate (pos. control)
• Chamber 3: DH5α + wt Shewie (neg. control)
• Chamber 4: DH5α/pUC19 + wt Shewie + Lactose
• Chamber 5: DH5α/pUC19 + wt Shewie + Lactate (pos. control)
• Chamber 6: DH5α/pUC19 + wt Shewie (neg. control)
• Chamber 7: wt Shewie + Lactose (neg. control)
• Chamber 8: DH5α + Lactose (neg. control)
• Chamber 9: DH5α/pUC19 + Lactose (neg. control)

Wednesday: August 13, 2008

RU1012 Light Repressible system experiment

• Streaked plates.
• All Cm/Amp plates

Dark

• Plate 1: RU1012 w/ plasmids
• Plate 2: RU1012
• Plate 3: Negative control (No bugs)

Light

• Plate 1: RU1012 w/ plasmids
• Plate 2: RU1012
• Plate 3: Negative control (No bugs)

Thursday: August 14, 2008

RU1012 Light Repressible system experiment

• Spread plates to try to create lawn.
• All Cm/Amp plates

Dark

• Plate 1: RU1012 w/ plasmids
• Plate 2: RU1012
• Plate 3: Negative control (No bugs)

Light

• Plate 1: RU1012 w/ plasmids
• Plate 2: RU1012
• Plate 3: Negative control (No bugs)

Friday: August 15, 2008

RU1012 Light Repressible system experiment

• Spread plates to try to create lawn.
• All Cm/Amp plates

Dark

• Plate 1: RU1012 w/ plasmids
• Plate 2: RU1012
• Plate 3: Negative control (No bugs)

Light

• Plate 1: RU1012 w/ plasmids
• Plate 2: RU1012
• Plate 3: Negative control (No bugs)