IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week14/Chemical and Light

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Mystery Colony PCR 10/08

Colony PCR of putative fungal colonies using primers BBpfx and mtrB500.

10-08 Mystery PCR MXHTA.jpg

Lane 1: 1 kb ladder

Lanes 2-6: P136 plate (500 bp)

Lane 7: P140 plate (1.9 kb)

Lanes 8-12: P122>131 plate (1.3 kb)

Colony PCR 10/11

Unadulterated gel

10-11 Colony PCR MXHTA.jpg


10-11 Colony PCR 1-12.jpg

Lanes 1-2: P148

Lane 3-4: P147 (picked 3)

Lanes 5-12: 147 (picked 6)

10-11 Colony PCR 13-24.jpg

Lanes 13-14: P147

Lanes 15-24: P146 (picked 17, 23)

10-11 Colony PCR 25-36.jpg

Lanes 25-26: P146

Lanes 27-36: P144 (picked 30, 35)

10-11 Colony PCR 37-48.jpg

Lanes 37-39: P144

Lanes 40-48: P145 (picked 41, 46)

Ligations 10/10

' Part 1 Part 2 Part 3 Notes ' Total Volume Growth
Ligation 1 123m4 ES 131 XP P1 EP ts in Kan just chem tran
8 4 3 15
Ligation 2 122m4 Es 131 XP P1 EP ts in kan just chem tran
8 4 3 15
Ligation 3 38+130 #20 ESX mmI P1 ES
2ul to tube 3
Ligation 4 39+130 #31 ESX P1 ES
10ul to insert tube 35
Ligation 5 142 Es P1 ES
10ul to insert tube 23
Ligation 6 138 #2 ES P1 ES
10ul to insert tube 20
Ligation 7 137 #32 ES P1 ES
10ul to insert tube 31
Ligation 8 140 ES P1 ES
10ul to insert tube 20
Ligation 9 131 XP 116 SP
4 4 8
Ligation 10 131 XP 117 SP
4 4 8
Ligation 11 131 XP 108 Sp
4 4 8