Transformations of P11, P15-20
|Name||Description||Origin||Size||Marker||Number of Colonies|
|P11||Light responsive system, dual regulation||pUC19-derived pMB1||4333||Amp/Cm||224|
|P15||GFP with Tet promoter||ColE1||937||Amp||360|
|P16||Tet repressible promoter||pMB1||54||Amp||264|
|P17||Inverter (TetR w/o promoter and Tet promoter)||OriS, P1 lytic, F1||902||Kan||2|
|P18||lambda promoter (cI regulated)||pMB1||49||Amp||112|
|P19||RFP with lambda promoter||pMB1||918||Amp||104|
|P20||GFP (LVA tagged) with Lac promoter||pMB1||1122||Amp, Kan||136|
6/23: P16, which has GFP under a constitutive Tet promoter did not appear to fluoresce (none of the fluorescent constructs did), so we restreaked all the of the plates (with the same antibiotic on which they were originally grown). They were left in the 37 degree incubator along with a blank Kan and a blank Amp plate (- controls).
6/23: The above was repeated in 5mL LB liquid cultures instead of plates.