IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week1/Light

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Transformations of P11, P15-20

Colony counts

6/23:

Name Description Origin Size Marker Number of Colonies
P11 Light responsive system, dual regulation pUC19-derived pMB1 4333 Amp/Cm 224
P15 GFP with Tet promoter ColE1 937 Amp 360
P16 Tet repressible promoter pMB1 54 Amp 264
P17 Inverter (TetR w/o promoter and Tet promoter) OriS, P1 lytic, F1 902 Kan 2
P18 lambda promoter (cI regulated) pMB1 49 Amp 112
P19 RFP with lambda promoter pMB1 918 Amp 104
P20 GFP (LVA tagged) with Lac promoter pMB1 1122 Amp, Kan 136


Restreaking

6/23: P16, which has GFP under a constitutive Tet promoter did not appear to fluoresce (none of the fluorescent constructs did), so we restreaked all the of the plates (with the same antibiotic on which they were originally grown). They were left in the 37 degree incubator along with a blank Kan and a blank Amp plate (- controls).

Overnight cultures

6/23: The above was repeated in 5mL LB liquid cultures instead of plates.