IGEM:Harvard/2006/vlau/Notebook/2006-7-19

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Pre-Working Stocks & Working Stocks

1. Rxn Mixtures

Description Plate Locations Total
c4.0.1: core barrel oligos box 4_A: row A (1-12), row B (1-12), row C (1-12), row D (1-12), row E (1-12), row F (1-6) 66
c4.0.2: core top lid oligos box 4_A: row F (7-12), row G (1-12), row H (1-12); box 4_B: row A (1-3) 33
c4.0.3: core bottom lid oligos box 4_B: row A (4-12), row B (1-12), row C (1-12) 33
c4.0.4: barrel oligos @ outside aptamers -aptamers box 4_B: row D (1-4) 4
c4.0.5: barrel oligos @ outside aptamers +aptamers box 4_B: row D (5-8) 4
c4.0.6: barrel oligos @ inside aptamers -aptamers box 4_B: row D (9-12) 4
c4.0.7: barrel oligos @ inside aptamers +aptamers box 4_B: row E (1-4) 4
c4.0.8: two-oligo latch sequences for barrel box 4_B: row E (5-8) 4
c4.0.9: four-oligo latch sequences for barrel box 4_B: row E (9-12) 4
c4.0.10: barrel oligos @ latches -latches box 4_B: row F (1-4) 4
c4.0.11: two-oligo latch sequences for top lid box 4_B: row F (5-6) 2
c4.0.12: two-oligo latch sequences for bottom lid box 4_B: row F (7-8) 2
c4.0.13: four-oligo latch sequences for top lid box 4_C: row A (1-2) 2
c4.0.14: four-oligo latch seequences for bottom lid box 4_C: row A (3-4) 2
c4.0.15: top lid oligos @ latches -latches box 4_C: row A (5-6) 2
c4.0.16: bottom lid oligos @ latches -latches box 4_C: row A (7-8) 2
c4.0.17: two-oligo latch barrel displacement oligos box 4_C: row A (9-12) 4
c4.0.18: two-oligo latch top lid displacement oligos box 4_C: row B (1-2) 2
c4.0.19: two-oligo latch bottom lid displacement oligos box 4_C: row B (3-4) 2
c4.0.20: four-oligo latch barrel displacement oligos box 4_C: row B (5-8) 4
c4.0.21: four-oligo latch top lid displacement oligos box 4_C: row B (9-10) 2
c4.0.22: four-oligo latch bottom lid displacement oligos box 4_C: row B (11-12) 2
c4.0.23: barrel zipper oligos box 4_C: row C (1-4) 4
c4.0.24: top lid zipper oligos box 4_C: row C (5-6) 2
c4.0.25: bottom lid zipper oligos box 4_C: row C (7-8) 2


Working Stocks Description Pre-Working Stocks Water Total
c4.0A no latches, no aptamers 1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL), 10 (4 μL), 15 (2 μL), 16 (2 μL) 39 μL 200 μL
c4.0B.core latch design 1, no aptamers 1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL) 47 μL 200 μL
c4.0B.latches latch design 1, no aptamers 8 (4 μL), 11 (2 μL), 12 (2 μL) - -
c4.0C.core latch design 2, no aptamers 1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL) 47 μL 200 μL
c4.0C.latches latch design 2, no aptamers 9 (4 μL), 13 (2 μL), 14 (2 μL), 23 (4 μL), 24 (2 μL), 25 (2 μL) - -


2. Protocol

   - mixed 10 μL of each 50 μM stock oligo to make pre-working stocks
   - mixed listed pre-working stocks to make working stocks where final concentration of each oligo is 250 nM