IGEM:Harvard/2006/Stock solutions

Salts

Calcium chloride

To make 100 mL of 0.10 M:

• 1.47 g CaCl₂ · 2H₂O
• add dH₂O to 100 mL

Magnesium chloride

To make 100 mL of 0.10 M:

• 2.03 g MgCl₂ · 6H₂O
• add dH₂O to 100 mL

Potassium chloride

To make 100 mL of 1.0 M:

• 7.46 g KCl
• add dH₂O to 100 mL

Sodium chloride

To make 100 mL of 1.0 M:

• 5.84 g NaCl
• add dH₂O to 100 mL

Standard buffers

Tris-acetate

To make 100 mL of 2.0 M:

• 24.2 g Tris
• add dH₂O to 90 mL
• add glacial acetic acid until desired pH (pH 7.4 is about 7 mL of acid)
• add dH₂O to 100 mL

Mixed buffers

Bock's selection buffer

To make 40 mL of 5x:

• 2 mL of 2 M Tris-acetate pH 7.4 (final concentration: 100 mM)
• 28 mL of 1 M NaCl (final concentration: 0.7 M)
• 1 mL of 1 M KCl (final concentration: 25 mM)
• 2 mL of 0.1 M CaCl₂ (final concentration: 5 mM)
• 2 mL of 0.1 M MgCl₂ (final concentration: 5 mM)
• 5 mL dH₂O

Reference:

1. Bock LC, Griffin LC, Latham JA, Vermaas EH, and Toole JJ. Selection of single-stranded DNA molecules that bind and inhibit human thrombin. Nature. 1992 Feb 6;355(6360):564-6. DOI:10.1038/355564a0 |

Bittker binding buffer

(buffer used by Bittker et al. in selecting for aptamer that binds streptavidin)

• 150 mM NaCl
• 50 mM Tris HCl pH 8.0
• 10 mM MgCl₂