IGEM:Harvard/2006/PEG precipitation
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PEG precipitation
- Make a stock solution of 20% PEG (w/v), 2.5 M NaCl.
- It may take a while to dissolve, but you can use a stir bar and low heat.
- For 10 mL solution: 2.0 g PEG, 1.46 g NaCl
- Add 0.25 volume of the PEG/NaCl solution to your folded nanostructure mix.
- Incubate on ice for 15 minutes
- Spin 16k rcf for 10 minutes
- Pipette out supernatant into separate tube
- Resuspend pellet in 1x folding buffer volume equal to the supernatant
- Run equivalent volumes of pellet and sup on 2% agarose gel (+ 11 mM MgCl2)
