IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-7-27
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Possible PEG precipitation titration
(if Val's doesn't come out, or if would like more precision...)
| trial | folding reaction (μL) | 20% peg / 2.5 M NaCl (μL) | water (μL) | final PEG concentration |
| 1 | 10 | 4 | 6 | 4% |
| 2 | 10 | 5 | 5 | 5% |
| 3 | 10 | 6 | 4 | 6% |
| 4 | 10 | 7 | 3 | 7% |
| 5 | 10 | 8 | 2 | 8% |
| 6 | 10 | 9 | 1 | 9% |
| 7 | 10 | 10 | 0 | 10% |
Gel analysis
| lane | contents |
| 1 | 1kb+ ladder |
| 2 | 10 μL nanostructures |
| 3 | trial 4 pellet |
| 4 | trial 4 supernatant |
| 5 | trial 5 supernatant |
| 6 | trial 5 pellet |
| 7 | trial 6 supernatant |
| 8 | trial 6 pellet |
| 9 | trial 7 supernatant |
| 10 | trial 7 pellet |
| 11 | trial 8 supernatant |
| 12 | trial 8 pellet |
| 13 | trial 9 supernatant |
| 14 | trial 9 pellet |
| 15 | trial 10 pellet |
results
- it's difficult to draw any firm conclusions because of the poor quality of the gel, but it appears that increased concentrations of PEG lead to increased precipitation
- where next: higher concentrations of PEG?
