Redo of yesterday's procedure
CIP of digested 011/sp
gel of post-CIP'd
- 1: 1kb+
- 2: undigested 11
- 3: empty
- 4: 11/sp marker
- 5: 11/sp (not shown)
- 6: 11/sp (not shown)
- 7: 11/sp (not shown)
- 8: 11/sp (not shown)
- 9: 11/sp marker
- 10: 1kb+
Very carcinogenic :D 40uL EtBr, 20min
gel purification and pcr purification
2000mg gel; purified into 6 tubes. sent all 6 tubes into 1 pcr purification. eluted in 30 eb.
- J36011 /SP + J36010 /XP (latest), respectively.
- BB vector: 70ng @ (70 ng/uL) = 1 uL
- BB insert: 3*(~1800/3700bp)*70ng=102.16 ng @ (20.2 ng/uL) = 5.06 uL
- 5x dilution: 2uL
- h20: 1.94uL
Sent 4 orders: 11 Vf2&vr and 22 vf2&vr, rush order.
Transformants plated at 8:00 PM, placed in incubator at 8:25 PM.