IGEM:Cambridge/2008/Notebook/Voltage/2008/08/11

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Kdp?

  • PCR colonies on plates - no insert seen.
  • Transform overweekend ligation.

OsmY and RBS

  • PCR B0030 and restrict 10μL with Xba.
  • Plasmid extract OsmY and restriction digest with Xba.
  • Ligate and transform cells with 5μL