Results of transformation from 23/08/2008
Vector |
Strain |
Antibiotic |
number of colonies |
Conclusion
|
ECE166 |
IA771, glycerol stock with medium B |
Cm5 |
one large colony and a lot of very small colonies |
Contamination?
|
Control |
IA771, glycerol stock with medium B |
Cm5 |
a lot of very small colonies |
Contamination?
|
ECE166 |
IA771, glycerol stock without medium B |
Cm5 |
many very small colonies |
Contamination?
|
Control |
IA771, glycerol stock without medium B |
Cm5 |
a few tiny colonies |
Contamination?
|
ECE166 |
IA751, fresh |
Cm5 |
9 large colonies, no small ones |
to test
|
Control |
IA751, fresh |
Cm5 |
no growth, scratches |
ok
|
ECE166 |
IA771, fresh |
Cm5 |
1 large colony, many small worm-like growth, scratches? |
to test
|
Control |
IA771, fresh |
Cm5 |
no colonies at all |
ok
|
ECE171 |
IA771, fresh |
Kan5 |
no colonies |
??
|
Control |
IA771, fresh |
Cm5 |
no colonies |
ok
|
ECE171 |
IA751, fresh |
Kan5 |
about 30 large colonies and 100 small colonies |
to test?
|
Control |
IA751, fresh |
Cm5 |
3 large colonies, about 20 small to medium colonies |
contamination?
|
ECE112 |
IA751, fresh |
Cm5 + Spc100 |
no growth |
??
|
Control |
IA751, fresh |
Cm5 + Spc100 |
no growth |
|
ECE153 |
IA751, fresh |
Spc50 |
about 500 medium to large colonies |
to test
|
Control |
IA751, fresh |
Spc50 |
no growth |
ok
|
|