IGEM:Cambridge/2008/Notebook/Bacillus/2008/08/20

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Check vector ECE190

- Plasmid miniprep

- Nanodrop

- Double digest : 6μL of SDW, 8μL of DNA, 1μL of buffer, 1μL of XbaI and 1μL of PstI

- Run on a gel

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- Result : 2 bands (lane3, a little bit more than 3000b and a little bit more than 5000b) :ok!