IGEM:Cambridge/2008/Notebook/Bacillus/2008/08/09

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Result from Erythromycin test

Problem : colonies on each plate

  • Reason : Erythromycin has to be diluted in ethanol, not water
  • To be sure, took a bacillus colony with no resistance and plated it onto Ery 0.5

On Monday we will remake the Erythromycin plates, and replate everything with the proper controls. Unfortunately, we do not have a positive control for erythromycin resistance, but if things die (i.e. negative control works), we should be in business.