IGEM:British Columbia/Protocols/Glycerol Stocks
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Materials
- 60% autoclaved glycerol
- Day/overnight culture
Equipment
- 1.5 microfuge tube/cryovial
Method
- Label each tube with: strain, date, Biobrick part number/ assembly number, antibiotic and any other identifying information
- Aseptically add 0.25mL 60% glycerol to the tube, then add 0.75mL of culture (final glycerol concentration 15%).
- Vortex briefly
- Store in -80°C freezer