IGEM:British Columbia/2009/Protocols/Glycerol Stocks

Making Glycerol Stocks

Supplies Needed:

• 60% autoclaved glycerol
• Day/overnight culture
• 1.5mL microfuge tube/cryovial

Steps:

1. Label the tube with: strain, date, BioBrick part number/plasmid or other identifying information, antibiotic (if necessary)
2. Aseptically add 0.25mL 60% glycerol to the tube, then add 0.75mL of culture (final glycerol concentration 15%).
3. Vortex briefly.
4. Put in -80°C freezer.