IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/19

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Sequencing Results

  • pBAD Strong, pBAD Weak1, pBAD Weak2, J23100+RBS, RBS-RFP-TERM miniprepped plasmids were sent to NAPS for sequencing using the VF2 and VR primers

pBAD Strong

  • Complete consensus for the pBAD sequence, all desired mutations are present
  • There is a mutation in the Biobrick prefix in the EcoRI restriction site. Both primer sequences show the same mutation

pBAD Strong1

  • Correct mutations are present
  • There is a mutation in the Biobrick prefix in the EcoRI restriction site. Both primer sequences show the same mutation
  • There is a significant number of unknown nucleotides in the sequence

pBAD Strong2

  • Correct mutations are present

J23100+RBS

  • Sequence failed - no signal

RBS-RFP-GFP

  • Weak signal
  • Sequence is correct