IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/19

iGEM Project name 1

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Sequencing Results pBAD Strong, pBAD Weak1, pBAD Weak2, J23100+RBS, RBS-RFP-TERM miniprepped plasmids were sent to NAPS for sequencing using the VF2 and VR primers pBAD Strong Complete consensus for the pBAD sequence, all desired mutations are present There is a mutation in the Biobrick prefix in the EcoRI restriction site. Both primer sequences show the same mutation pBAD Strong1 Correct mutations are present There is a mutation in the Biobrick prefix in the EcoRI restriction site. Both primer sequences show the same mutation There is a significant number of unknown nucleotides in the sequence pBAD Strong2 Correct mutations are present J23100+RBS Sequence failed - no signal RBS-RFP-GFP Weak signal Sequence is correct