IGEM:Arizona State 2014Literature

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Research Articles

Fatty Acid Synthesis

  1. (2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Eric J. Steen, Yisheng Kang, Gregory Bokinsky, Zhihao Hu, Andreas Schirmer, Amy McClure, Stephen B. del Cardayre and Jay D. Keasling. Nature. 275: 28593-28598. [1]
    Summary: This group used TES in their system. Figure 2 shows that TES works best in cells in which a gene called fadD or fadE has been deleted. This is something to consider.

  2. (2000) Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fatty Acid Biosynthesis in Escherichia coli. Mark S. Davis, José Solbiati and John E. Cronan Jr. J Biol Chem. 275: 28593-28598. [2]
    Summary: The authors expressed the four subunits of the E. coli acetyl-CoA carboxylase on a single plasmid. Very promising indicator for success!

Cell-cell communication

  1. (2009) Engineering multicellular systems by cell–cell communication. Anand Pai, Yu Tanouchi, Cynthia H Collins, and Lingchong You. Curr Opin in Biotech. 20:461–470. [3]
    Summary: Overview of using cell-cell communication in synthetic systems.

  2. (2007) Engineered bidirectional communication mediates a consensus in a microbial biofilm consortium. Katie Brenner, David K. Karig, Ron Weiss, and Frances H. Arnold. PNAS. 20:461–470. [4]
    Summary: This group engineered cells to communicate with each other. The signaling flows from cell A to B and from B to A.

Wax ester synthase

  1. (2005) The Wax Ester Synthase/Acyl Coenzyme A:Diacylglycerol Acyltransferase from Acinetobacter sp. Strain ADP1: Characterization of a Novel Type of Acyltransferase Tim Stöveken, Rainer Kalscheuer, Ursula Malkus, Rudolf Reichelt, and Alexander Steinbüchel, J Bacteriol. Feb 2005; 187(4): 1369–1376. [5]
    Summary: This group was able to take a wax ester synthase and CoA synthase from Acinetobacter and use it in E. Coli. The plasmid that was used still needs to be located.