Cell Picking
- From the transformations of the previous day, the positive control (Tet) and one plate (Plate 4 with 6μL) had growth on them
- Plate 1: 1 μl of Tet with DNA (positive control) GROWTH
- Plate 2: 1μl of ligation mixture (tes and rbs) NO GROWTH
- Plate 3: 3 μl of ligation mixture NO GROWTH
- Plate 4: 6 μl of ligation mixture 3 COLONIES
- Plate 5: 1 μl RBS Backbone (negative control) NO GROWTH
- Plate 6: 3 μl RBS backbone NO GROWTH
- Plate 7: 6 μl RBS Backbone NO GROWTH
- Warmed up lb in shaker and picked 3 colonies from plate 4 to be put for growth at 10:45 am. Each tube had 5mL of LB and a colony
Mini prepped one of the EtOH tubes that was in the shaker that seemed to have more growth than the other two. Labelled it EtOH 1
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