IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/07

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Gel Extraction

  • Ran gel extraction on gel from 7/4

Procedure:

  • Cut selected fragments out from the gel
  • Dissolve in ADB buffer. For the buffer, 3x the volume (measured by weight) of the gel was added. Place on a heat block to dissolve

Issues with the gel indicate that one or more enzymes may not be functioning correctly.

Enzyme Testing:

  • Tested enzymes with MCherry
  • Ran digests with single cuts for X P S and E on MCherry as well as MCherry with cuts on X and P, E and S, and X and S, and E and S.
  • Let run overnight