IGEM:American University/2009/Notebook/Ecosystem Study/Entry Base

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Day 1: Examining the Marsh for the First time.

Location: American University, to the right of the Kogod Business school near Massachusetts Ave.

Picture: File:/Users/brittneythompson/Desktop/Marsh.png]

Abiotic Factors: -Rocks -Dirt -Light -Water -Sewage drain off to the left

Biotic Factors: -Cardinal Flower (upper right) -Grass -Moss -Clovers -Butterfly bus (upper right) -Cat tails (lower center)

Day 2: Hay Infusion Culture

Observations: The water has become brown and there is a thin layer of mold on top. The sample smells of manure and swampy smell.

Sample 1: Taken from the top layer of the culture 1. 100 um, oblong, floats, pouches within membrane, dark colored 2. 450 um, pink colored, long stripes present within membrane, thin stick like. 3. 70 um, green colonies, 7 large colonies surrounded by several smaller ones, non-moving

Sample 2: Taken from the bottom layer of the culture 1. 50-60 um, dark colored, moves fast, vacuoles visible within membrane 2. 20-30 um, changes shape, floats, colorless 3. 60 um, fast, oval shape, colorless, one large vacuole visible

If the Hay culture were to grow for another two months I believe there would be a growth in some of the bacteria currently present. However, the number will stop after the ecosystem reaches its carrying capacity for that specific niche of the marsh. I chose bacteria to grow best in this environment because of their small size and relative ability to live in countless places, they will have the most fitness in the culture. Other creatures are bound to run out of resources before the bacteria, and soon the bacteria will hold majority due to the selective pressures.