Hygromycin B

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Hygromycin B is an antibiotic used to select prokaryotic and eukaryotic cells. It works by interfering with protein synthesis. Cloning of the resistance genes and fusion with eukaryotic promoters has resulted in vectors to select for resistance in both prokaryotic and eukaryotic cells.

Mode of action

Interferes with protein synthesis.

Mechanism of resistance

Resistance genes are kinases that inactivate Hygromycin B through phosphorylation.

Stock solution

  • Stock Solution Concentration: 50 mg/mL
  • Solvent: H2O


sensitive to high concentrations of acid, but short-term exposure to dilute acids tolerated;

Usage notes

The working concentration for selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for resistant cells varies between 25 and 1000 μg/ml. You need to determine your optimum concentration experimentally. For some typical values by cell types see the table below.

typical HygB concentrations by cell type
cell type HygB concentration
mammalian cells 200 μg/ml
plant cells & bacteria 20-200 μg/ml
fungi 200-1000 μg/ml

For more information on working concentrations: [1]

External links

Wikipedia entry: Hygromycin B

Information from hygromycin.net (Invivogen)

  • mechanism of action: [2]
  • resistance genes: [3]
  • chemistry, stability: [4]