HighPoint/CannonLab:UV-Vis Spectrometry

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   Research=#,Protein Membrane=Protein Membrane Interactions, Osmolytes=Osmolyte Effects
   Protocols=#, Vesicles=Lipid Vesicle Preparation, Solutions=Buffer and Osmolyte Solutions, Fluorescence=Fluorescence Spectrometry, UV-Vis=UV-Vis Spectrometry, Pipetting=Pipetting Exercise
   Notebooks=#, Master List=Notebook, Dr Cannon=Notebook/Jonathan Cannon
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UV-Vis Spectrometry

Using the Shimadzu UV-Vis Spectrometer


  1. Open the LCD screen on the spectrometer
  2. Turn ON the spectrometer
  3. Wait for >3.5 minutes for the instrument to initialize
  4. If initialization stalls, turn OFF the spectrometer and repeat until the spectrometer completes the checklist (2-3 times is typical)
  5. Press F4 on the spectrometer to turn control over to the PC
  6. At any time turn ON the computer
  7. Open the software
  8. On the menu option "Configure > Utilities" make sure "spectrophotometer ON" is checked


Wavelength Scans

Kinetic Measurements