Helpful Websites

• National Center for Biotechnology Information

Home Page

• Parts Registry of BioBricks

Prefix and Suffix Help

• IDT Integrated DNA Technologies- Check for %GC (40-60) and Melting Temp. (52-68C)

Analyze Your Sequence

• NUPACK Nucleic Acid Package- check both primers for hairpins and trouble spots (No Red!!)

Analzye both Primers

• Ape- A Plasmid Editor- Useful tool for checking sequences and finding reverse complements

Download here