10/17/14 - Jonah Thomas
- Performed transfections for KAH60/pcVn plasmids
HEK293 Transfection w/ KAH60/pcVn
- The Haynes Lab protocol for transfections was followed
Note: The plasmid concentration used for this transfection was ~58ng/μL meaning the total plasmid amount used to transfect each well was ~1160ng
- A total of 2 wells were transfected with the KAH60/pcVn plasmids
- Two mock transfections were also completed as a control for each plate
- In order to improve plasmid uptake during the transfection, one plate (Plate 2) was spun in a centrifuge and Plate 1 was left to incubate as a control
The results of this transfection with be observed and reported on Monday, October 20, 2014.
Plan of Action: Week of 10/20/14
- Monday (10/20): Record and report the results of the transfection mini-experiment. Also, perform transduction with E. coli for new plasmid mini preps
- Tuesday (9/23): Check bacterial colonies and incubate cell colonies in LB broth for new mini preps
- Wednesday (9/24): Perform 4 minipreps
- Thursday (9/25): Perform new set of transfections
- Friday (9/26): Check status of transfections
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