Haynes Lab:Notebook/Shawn Cloning bootcamp/2015/02/19

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Entry title

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 .4355 1.901 435.2575
2. Plasmid 2 .186 1.9245 186.0375

OD260/280 measures the purity if the sample which should be around 2.
ng/μL measures how much product was yielded.


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = 1500
2. Plasmid 2 = 4500
Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'




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