Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/09/02

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Owwnotebook icon.pngU-2 OS Transfection, Day 1 Report.pngMain project page
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Overview

  • Photographing U-2 OS plate (day 1 & day 2 samples) using phase & mCherry
  • Harvesting U-2 OS day 1 samples (2 biological replicates, 1 negative control)
    • flow cytometry of day 1 samples
    • TRIzol digestion of cells and storage at -80 °C

Fluorescence Microscopy

Well 1
2015-09-02 Well 1 KAH126-MV2 phase mCherry 10x.jpg

Well 2
2015-09-02 Well 2 KAH126-MV2 phase mCherry 10x.jpg

Well 3
2015-09-02 Well 3 no plasmid phase 10x.jpg

Well 4
2015-09-02 Well 4 KAH126-MV2 phase mCherry 10x.jpg

Well 5
2015-09-02 Well 5 KAH126-MV2 phase mCherry 10x.jpg

Well 6
2015-09-02 Well 6 no plasmid phase 10x.jpg


Flow Cytometry

Well 1 (KAH126-MV2)
2015-09-02 Well 1 FC.png


Well 2 (KAH126-MV2)
2015-09-02 Well 2 FC.png


Well 3 (no plasmid)
2015-09-02 Well 3 FC.png


Results

Transfection efficiency as measured by flow cytometry is ~40% for replicate A in Well 1, and ~60% for replicate B in Well 2. Total cell count is approximately 400,00 cells for Wells 1 and 2 and approximately 800,000 cells in Well 3.