Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/09
|K562 Transfection Results & RNA Miniprep||Main project page|
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Took fluorescence microscopy images & flow cytometry measurements of all 6 samples today. Harvesting the first three samples for RNAseq. Following the protocol for RNA miniprep found here.
Well 1: GFP (post 24-hr sample)
Well 2: KAH126-MV2 (post 24-hr sample, rep 1)
Well 3: KAH126-MV2 (post 24-hr sample, rep 2)
Well 4: GFP (post 48-hr sample)
Well 5: KAH126-MV2 (post 48-hr sample, rep 1)
Well 6: KAH126-MV2 (post 48-hr sample, rep 2)
Follow the instructions given here. Samples are stored at -80 °C.