Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/10
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K562 Transfection Results & RNA Miniprep | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OverviewDay 2 of collecting flow cytometry data, fluorescence microscopy data, and harvesting cells for RNA. Also need to set up two more samples for a "no plasmid" control transfection. Current cell density of K562 culture is 420,000 cells/mL. Need to wait until culture is around 800,000 cells/mL before proceeding with mock transfection. Following the protocol for RNA miniprep found here. Fluorescence MicroscopyWell 4: pMaxGFP, post-48hr Well 5: KAH126-MV2, post-48hr Well 6: KAH126-MV2, post-48hr Flow Cytometry
RNeasy / TrizolSamples are stored at -80 °C.
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