Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/06/22

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.pngPlasmid miniprep of g032, g034, g044, g048 in pX330g <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Overview

René built several CRISPR/gRNA plasmids with a GFP reporter in the pX330 plasmid. I'll be making some more of those plasmids using the plasmid miniprep kit from Sigma.

Started 5 mL cultures of g032, g034, g044 and g048 using DH5α-T in LB-Amp. Cultures are shaking overnight at 37 °C.