Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/06/22

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Owwnotebook icon.pngPlasmid miniprep of g032, g034, g044, g048 in pX330g Report.pngMain project page
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Overview

René built several CRISPR/gRNA plasmids with a GFP reporter in the pX330 plasmid. I'll be making some more of those plasmids using the plasmid miniprep kit from Sigma.

Started 5 mL cultures of g032, g034, g044 and g048 using DH5α-T in LB-Amp. Cultures are shaking overnight at 37 °C.