Haynes Lab:Notebook/Engineering PC-TFs/2015/02/04

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Summary Digest and Gel Verification Reagent Volume Expected: BL09: 55081.78Da 15 μL/lane; 1% agarose; Observed lengths: W1: Page Ruler Plus Prestained W2: Purified BL09 W3: Blank 11 W4: BL09: U2OS Cell Lysate W5: Blank W6: Precision Plus Protein Dual Color Standard ladder plus prestained.jpg Left Ladder Plus Protein™ Dual Color Standards -161-0374.jpg 4X NuPAGE Dye 5.0 μL 1M DTT 1.0 μL Protein Prep Buffer 4.0 μL Protein Sample 10.0 μL dH2O 0.0 μL Total 20 μL --> 100°C/ 5 min. Observed: Possible PcTF band ~ 60kDa. PcTF reported to be larger in observation than theoretical size.