Haynes Lab:Notebook/Engineering PC-TFs/2015/02/04

From OpenWetWare
Jump to: navigation, search

Hayneslab3.gif
Asu logo 3.gif

Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Summary

Digest and Gel Verification

Reagent Volume

Expected:
BL09: 55081.78Da

Today's gel

15 μL/lane; 1% agarose;
Observed lengths:
W1: Page Ruler Plus Prestained
W2: Purified BL09
W3: Blank 11
W4: BL09: U2OS Cell Lysate
W5: Blank
W6: Precision Plus Protein Dual Color Standard

ladder plus prestained.jpg Left Ladder Plus Protein™ Dual Color Standards -161-0374.jpg

4X NuPAGE Dye 5.0 μL
1M DTT 1.0 μL
Protein Prep Buffer 4.0 μL
Protein Sample 10.0 μL
dH2O 0.0 μL
Total 20 μL --> 100°C/ 5 min.


Observed: Possible PcTF band ~ 60kDa. PcTF reported to be larger in observation than theoretical size.