Haynes Lab:Notebook/Engineering PC-TFs/2015/01/26

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Summary Gel Extraction Reagent Volume Expected: BL05 [XbaI/SpeI] bl05 insert: 2630 v0120 vector: 1538, 6185 XbaI cut CMV/MV9: 5197 CMV/MV9 with [XbaI] 5197 30μL/lane; 1% agarose; Observed lengths: W2 BL05 S1 W3 BL05 S2 W4 CMV/MV9 S1P(urple) W5 CMV/MV9 S1B(lue) Ladder DNA[BL05: W2,W3*](CM9: W4,W5) [8.0 μL] (5.0 μL) 10X buffer 3.0 μL XbaI 1.0 μL SpeI 1.0 μL (0uL) dH2O [17μL] (21μL) Total 30 μL --> 37°C/ 30 min. BL05 Gel Purification and CMV/MV9 XbaI Cut Vector Concentrations