Reagent
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Volume
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Expected:
BL01/5 Ligation Colonies [XbaI/NruI]
Forward insertion: 710, 7000
Reverse insertion: 3238, 4528
No insertion: 710, 4490
CMV/MV9 with [XbaI/NruI]
710, 4490
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15 μL/lane; 1% agarose;
Observed lengths:
W2 BL05 LCR SV1 (small volume lcr reaction product: 4uL)
W3 BL05 LCR SV2
W4 BL05 LCR LV1 (large volume lcr reaction product: 16uL)
W5 BL05 LCR LV2
W6 BL01 LCR SV1
W7 BL01 LCR SV2
W8 Empty CMV/MV9
W9 BL01 LCR LV1
W10 BL01 LCR LV2
W11 LCR Negative Control Colony
W12 BL05 Traditional Ligation SV1
W13 BL05 Traditional Ligation SV2
W14 BL05 Traditional Ligation LV1
Ladder
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