Haynes Lab:Notebook/Engineering PC-TFs/2014/10/13

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Summary

  • RFP
  • Bradford

Plate reader measurements

RFP signal

  1. U2OS BL09 = 181
  2. Mock Transfection = 25


Bradford assay

  1. Standard 1 (0 μg) = 0.276
  2. Standard 2 (1 μg) = 0.234
  3. Standard 3 (2 μg) = 0.394
  4. Standard 4 (4 μg) = 0.443
  5. Standard 5 (8 μg) = 0.644
  6. Standard 6 (16 μg) = 0.829
  7. U2OS BL09 = 0.313
  8. Mock Transfection = 0.302


Standard curve

Bradford rfp.png


Final results:

Sample Protein μg/μL Protein*100 μL Raw Fluor. (100 uL) Fluor/ μg protein
U2OS BL09 0.765 76.50 181 2.365929
Mock Transfection 0.464 46.45 25 0.538235