Haynes Lab:Notebook/Engineering PC-TFs/2014/05/20

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Summary Concentration of regrown BL01 colonies one and two (C1 and C2) eluted with 100μL dH2O. Plasmid OD260 OD260/280 ng/μL 1. Purified BL01 C1 --- 1.942 37.189 1. Purified BL01 C2 --- 1.922 29.894 Restriction Digest Table Reagent Volume Expected: 1. BL01 = 2500bp DNA(BL01 C1) 25.0 μL 10X buffer 3.0 μL XbaI 1.0 μL SpeI 1.0 μL dH2O 0 μL Total 30 μL --> 37°C/ 35 min. Gel Purification: BL01/V0120 Using Prior Digest Placed 15μL 1kB Ladder in well one and 30μL of BL01 digest in well two. Ran gel at 110V for 55 minutes. Placed gel on UV transilluminator and cut out desired band. Fail.