Summary
- Using colony one of previously constructed CMV/MV9.
DNA(CMV/MV9) Concentration: 163ng/μL |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
dH2O |
7.5 μL
|
Total |
15 μL --> 37°C/ 15 min.
|
Mixture from Above Digest |
15.0 μL
|
10X Phosphotase buffer |
2.0 μL
|
Phosphotase |
1.0 μL
|
dH2O |
2.0 μL
|
Total |
20 μL --> 37°C/ 10 min. 75°C for 2 min.
|
- DNA Purification Kit standard protocol. Used 600μL binding buffer solution.
Plasmid |
OD260 |
OD260/280 |
ng/μL
|
1. Purified DNA |
0.059 |
2.046 |
59.108
|
- Eluted with 15μL (-1μL for concentration reading) H2O and put in Cameron box labeled Dephos XbaI CMV/MV9 5/12/14
|