Haynes Lab:Notebook/Engineering PC-TFs/2013/09/21

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September 21, 2013 Although there was no pellet, ran a DNA Clean & Concentrator on samples. Add 2 volumes of DNA Binder Buffer to each DNA sample (200 μL). Place mixture in spin column into 2 ml collection tube. Centrifuge at full speed for 30 seconds. Add 20 μL of DNA Wash Buffer and centrifuge for 30 seconds. Repeat this step. Place Zymo-Spin Column into a new 1.5 ml tube. Add 20 μL of water directly to column matrix and spin to elute DNA. Ran samples on agarose gel. Gel is a day old, probably not an accurate testing to see if PEG/MgCl2 method works.