Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/01/15

Project name

Main project page Previous entry      Next entry

01/15/2016 Next steps: PCR-Purify gB015 Sample Read# Location 260 Raw 280 Raw 320 Raw 260 280 260/280 ng/µL gB015 C3 0.055 0.048 0.041 0.008 0.004 1.714 7.573 The gel showed a fragment at 500bp, but the gBlock is only 120bp. The PCR-purification also resulted in a low yield. I will probably PCR-optimize gB015 before continuing with ligation. Ligate gB015 and LasR_MRV backbone (Moved to a later date)