Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/01/15

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01/15/2016

Next steps:

PCR-Purify gB015
Sample Read# Location 260 Raw 280 Raw 320 Raw 260 280 260/280 ng/µL
gB015 C3 0.055 0.048 0.041 0.008 0.004 1.714 7.573
The gel showed a fragment at 500bp, but the gBlock is only 120bp. The PCR-purification also resulted in a low yield. I will probably PCR-optimize gB015 before continuing with ligation.
Ligate gB015 and LasR_MRV backbone (Moved to a later date)