Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/06/15

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06/15/2015

Grew liquid cultures for the following colonies:
BjaI: 1, 3, 4
CerI: 2
EsaI: 1 through 6
LasI: 3
RhlI was unsuccessful so I didn't grow any liquid cultures for that plasmid.

Digested RhlI (from the PCR optimization done without DMSO) with E + X. Ran another ligation for RhlI + digested MSV. Fast-transformed.