Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/06/15

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


Grew liquid cultures for the following colonies:
BjaI: 1, 3, 4
CerI: 2
EsaI: 1 through 6
LasI: 3
RhlI was unsuccessful so I didn't grow any liquid cultures for that plasmid.

Digested RhlI (from the PCR optimization done without DMSO) with E + X. Ran another ligation for RhlI + digested MSV. Fast-transformed.